According to investigators, a key reason for the link may lie in circadian “clocks,” the molecular machinery within every cell of every organism, which have evolved to adapt to daily stresses, such as changes in light and temperature caused by the rising and setting of the sun.
A team of investigators at the Buck Institute have unveiled a link between eye health, diet, circadian rhythms and lifespan in Drosophila.
According to a news release from Buck Institute, the study was published in a recent issue of Nature.1
The news release noted that previous studies have shown in humans that there is an association between eye disorders and poor health.
“Our study argues that it is more than correlation: dysfunction of the eye can actually drive problems in other tissues,” said Pabjaj Kapahi, PhD, a lead author of the study. “We are now showing that not only does fasting improve eyesight, but the eye actually plays a role in influencing lifespan.”
“The finding that the eye itself, at least in the fruit fly, can directly regulate lifespan was a surprise to us,” co-lead author, Brian Hodge, PhD, who did his postdoctoral studies in Kapahi’s lab, said in the news release.
According to Hodge, a key reason for the link may lie in circadian “clocks,” the molecular machinery within every cell of every organism, which have evolved to adapt to daily stresses, such as changes in light and temperature caused by the rising and setting of the sun. These 24-hour oscillations - circadian rhythms - affect complex animal behaviors, such as predator-prey interactions and sleep/wake cycles, down to fine-tuning the temporal regulation of molecular functions of gene transcription and protein translation.
According to the institute’s news release, in 2016 Kapahi’s lab published a study in Cell Metabolism showing that fruit flies on a restricted diet had significant changes in their circadian rhythms in addition to extending lifespan.
When Hodge joined the lab later that year, he wanted to dig deeper to figure out which processes that enhance circadian functions were altered by the diet change, and whether circadian processes were required for the longer lifespan seen with dietary restriction.
“The fruit fly has such a short lifespan, making it a really beautiful model that allows us to screen a lot of things at once,” Hodge, who is currently a scientist at Fountain Therapeutics in South San Francisco, said in the news release.
The study began with a broad survey to see what genes oscillate in a circadian fashion when flies on an unrestricted diet were compared with those fed just 10 percent of the protein of the unrestricted diet.
Hodge quickly noticed numerous genes that were both diet-responsive and also exhibiting ups and downs at different time points, or “rhythmic.” He then discovered that the rhythmic genes that were activated the most with dietary restriction all seemed to be coming from the eye, specifically from photoreceptors, the specialized neurons in the retina of the eye that respond to light.
This finding led to a series of experiments designed to understand how eye function fit into the story of how dietary restriction can extend lifespan. For example, they set up experiments showing that keeping flies in constant darkness extended their lifespan.
“That seemed very strange to us,” Hodge said in the release. “We had thought flies needed the lighting cues to be rhythmic, or circadian.”
They then used bioinformatics to ask: Do the genes in the eye that are also rhythmic and responsive to dietary restriction influence lifespan? The answer was yes they do.
“We always think of the eye as something that serves us, to provide vision,” Kapahi, also an associate adjunct professor of urology at UCSF, said in the news release.
Since the eyes are exposed to the outside world, Kapahi noted in the release, the immune defenses there are critically active, which can lead to inflammation, which, when present for long periods of time, can cause or worsen a variety of common chronic diseases. Additionally, light in itself can cause photoreceptor degeneration which can cause inflammation.
Kapahi also noted that extended time viewing computer and phone screens and being exposed to light pollution well into the night are conditions very disturbing for circadian clocks.
“It messes up protection for the eye and that could have consequences beyond just the vision, damaging the rest of the body and the brain,” he said in the release.
There is much to be understood about the role the eye plays in the overall health and lifespan of an organism, including: how does the eye regulate lifespan, and does the same effect apply to other organisms?
A key question posed by the work as it could apply to humans is whether photoreceptors in mammals affect longevity?
Hodge pointed out that it is probably not as much as in fruit flies, since a large portion of the energy in a fruit fly is devoted to the eye. But since photoreceptors are just specialized neurons, he said, “the stronger link I would argue is the role that circadian function plays in neurons in general, especially with dietary restrictions, and how these can be harnessed to maintain neuronal function throughout aging.”
Once investigators understand how these processes are working, they can begin to target the molecular clock to decelerate aging, Hodge noted, adding that it may be that humans could help maintain vision by activating the clocks within our eyes.
“It might be through diet, drugs, lifestyle changes … A lot of really interesting research lies ahead,” he concluded.
Pabjaj Kapahi, PhD; Brian Hodge, PhD; et.al; Dietary restriction and the transcription factor clock delay eye aging to extend lifespan in Drosophila. Nature, published June 2022. DOI: 10.1038/s41467-022-30975-4